生物学杂志

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高压均质法提取药用真菌多糖的研究

  

  1. 1. 江南大学 药学院 粮食发酵工艺与技术国家工程实验室, 无锡 214122;2. 江苏神华药业有限公司 江苏省药用真菌生物工程技术研究中心, 淮安 211600
  • 出版日期:2018-08-18 发布日期:2018-08-18
  • 通讯作者: 许正宏,博士,教授,主要从事发酵工程及生物制药相关研究,E-mail: zhenghxu@jiangnan.edu.cn
  • 作者简介:龚劲松,博士,副教授,研究方向为生物制药,E-mail: jinsonggong.bio@hotmail.com
  • 基金资助:
    国家自然科学基金项目(31401931)

The investigation on extraction of fungal polysaccharide via high-pressure homogenization strategy

  1. 1. National Engineering Laboratory for Cereal Fermentation Technology, School of Pharmaceutical Science,Jiangnan University, Wuxi 214122; 2. Medicinal Fungi Biotechnology Research Center of Jiangsu Province, Jiangsu Shenhua Pharmaceutical Co. Ltd., Huai′an 211600, China
  • Online:2018-08-18 Published:2018-08-18

摘要: 真菌多糖是一类重要的生物活性物质,具有多种功效,开展真菌多糖的分离提取研究,是进行后续产品开发的基础。采用高压均质法提取猴头菌(Hericeum erinaceus)胞内多糖,与传统的热水法和超声法进行比较,结果表明,通过高压均质法提取猴头菌胞内多糖显著优于传统的热水及超声提取方法,提取得率可达到16.42%;高压均质提取法提取所得猴头胞内多糖的总糖为57.11%,与热水提取法(58.45%)和超声提取法(60.23%)相当;蛋白检测结果显示高压均质提取法、热水提取法和超声提取法所得样品蛋白含量分别为26.34%、15.45%和10.12%;高压均质和超声提取所得的胞内多糖的分子量分布较热水提取法偏小;红外分析结果表明不同方法提取的多糖的红外光谱图出峰位置和峰形基本一致。

关键词: 高压均质法, 真菌多糖, 猴头菌, 分离提取

Abstract:  Fungal polysaccharides are a kind of important bioactive substances, which exhibit several positive effects on human. Extraction of fungal polysaccharide is the basis for further development of products. This study employed the high-pressure homogenization strategy for extraction of intracellular polysaccharide from Hericeum erinaceus, comparing with the conventional methods of hot water extraction and ultrasonic extraction. The experimental results showed that the polysaccharide yields by high-pressure homogenization reached 16.42%, which exhibited significant advantages over hot water extraction and ultrasonic extraction. The total sugar contents via the three methods were 57.11%, 58.45% and 60.23%, respectively; whereas the protein contents were 26.34%, 15.45% and 10.12%, respectively. The molecular weight of polysaccharides by high-pressure homogenization and ultrasonic extraction was relatively lower than that of hot water extraction. The FT-IR spectra analysis revealed that the peak shape and peak position of polysaccharides by various methods were in concordance with each other.

Key words: high-pressure homogenization, fungal polysaccharides, Hericeum erinaceus, extraction