生物学杂志

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利用GT1-7细胞体外研究雌激素对GnRH分泌及其相关基因表达的影响

  

  1. 1. 甘肃农业大学 动物科学技术学院, 兰州 730070;2. 北京市农林科学院 畜牧兽医研究所, 北京 100097
  • 出版日期:2018-04-18 发布日期:2018-04-18
  • 通讯作者: 郑琛,副教授,硕士生导师,研究方向为反刍动物营养,E-mail:zhengc@gsau.edu.cn;冯涛,副研究员,硕士生导师,研究方向为动物生殖的营养调控,E-mail: fengtao_gs@163.com
  • 作者简介:陈志龙,硕士研究生,研究方向为动物营养与饲料科学,E-mail: judy.happy@outlook.com
  • 基金资助:
    国家自然科学基金(31501950);北京市科技新星计划项目(Z141105001814046)

The effect of 17β-estradiol on GnRH secretion and GnRH-related gene expression using GT1-7 cells in vitro

  1. 1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070;2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
  • Online:2018-04-18 Published:2018-04-18

摘要: 雌激素对GnRH神经元活性及其合成分泌具有重要的调控作用。为了体外模拟雌激素对GnRH合成分泌的抑制作用,使用1 μmol/L的雌激素处理GT1-7细胞0、6、12、18、24和30 h,分别检测GnRH的分泌及相关基因GnRH、ERα、KISS-1和GPR54的表达。结果显示:雌激素处理GT1-7细胞GnRH分泌量相对于对照组先升高(6和12 h,P>0.05)后极显著降低(18和30 h,P<0.01)。雌激素处理组GnRH mRNA 表达在整个培养期间相对于对照组极显著降低(P<0.01),ERα(12和18 h,P<0.01)、KISS-1(12 h,P<0.01)和GPR54表达也极显著降低(6、12和24 h ,P<0.01)。结果表明:1 μmol/L雌激素能够抑制GT1-7细胞GnRH的分泌,可能是通过降低ERα、GnRH、KISS-1及GPR54等基因的表达来实现的。

关键词: 雌激素, GT1-7, GnRH, 基因表达

Abstract: Estrogens play essential roles in the neuroendocrine control of reproduction. To simulate inhibiting effects of GnRH secretion and GnRH-related genes(GnRH, ERα, KISS-1 and GPR54) expression by estrogens in vitro, the GnRH-secreting neuronal cell line GT1-7 was exposed to 1 μmol/L estradiol concentrations for 0, 6, 12, 18, 24 and 30 h, respectively. The result showed that GnRH secretion of GT1-7 cells treated with 1 μmol/L 17β-estradiol was decreased significantly(18 and 30 h, P<0.01), although slightly increased(6 and 12 h, P>0.05) at the beginning of the process. Compared with the control, GnRH mRNA treated with 1 μmol/L estradiol was significantly decreased(P<0.01) through all culture period. ERα(12 and 18 h, P<0.01), KISS-1(12 h, P<0.01) and GPR54(6, 12 and 24 h, P<0.01) mRNA were also decreased. It revealed that 1 μmol/L estradiol could inhibit GnRH secretion of GT1-7 cells and this effect may be achieved by down-regulating mRNA of ERα, KISS-1, GnRH and GPR54.

Key words: 17β-estradiol, GT1-7, GnRH, gene expression