生物学杂志

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一株耐锌菌株的分离鉴定及其生长特性研究

  

  1. 河西学院 农业与生物技术学院 甘肃省河西走廊特色资源利用重点实验室, 张掖 734000
  • 出版日期:2017-12-18 发布日期:2017-12-18
  • 通讯作者: 雷玉明,教授,研究方向为应用微生物学,E-mail:zyymlei@126.com
  • 作者简介:曹 礼,副教授,博士,研究方向为环境微生物学,E-mail:caolicl@126.com
  • 基金资助:
    河西走廊特色资源利用重点实验室项目(No. XZ1401)

Isolation and identification of a zinc-resistance strain and research on its growth characteristics

  1. College of Agriculture and Biotechnology; Key Laboratory of Hexi Corridor Resourses Utilization of Gansu, Hexi University, Zhangye 734000, China
  • Online:2017-12-18 Published:2017-12-18

摘要: 从造纸厂排污口的土壤中分离对锌具有较强耐受性的菌株HXZ-1。菌株HXZ-1能够在含锌离子浓度为50 mg/L的液体培养基中生长良好,能耐受锌离子的最高浓度为1000 mg/L。结合其生理生化特征和16S rRNA基因系统发育分析,将该菌株鉴定为原小单孢菌属(Promicromonospora sp. HXZ-1)。菌株HXZ-1最适培养条件为:锌离子浓度为50 mg/L、葡萄糖为碳源、蛋白胨为氮源。当锌离子浓度为50 mg/L时,菌株HXZ-1对锌吸附率最大,为29.1%;当锌离子浓度为500 mg/L时,培养时间为10 h时,菌株HXZ-1吸附率最大,为18.8%。菌株HXZ-1是一株对锌有较强抗性和吸附性的细菌,为重金属污染的微生物修复提供参考。

关键词: 耐锌, 16S rRNA, 原小单孢菌属, 生长特性

Abstract: A bacterium with strong tolerance to zinc, strain HXZ-1, was isolated from sewage of a paper mill. It could tolerate 1000 mg/L of zinc ion. Based on the phylogenetic analysis of the 16S rRNA gene and the physiologic & biochemical characteristics, it was identified as Promicromonospora sp. HXZ-1. The culture conditions of strain HXZ-1 were zinc ion concentration of 50 mg/L, glucose as carbon source and peptone as the nitrogen source. When the concentration of zinc ions in cultural liquid was 50 mg/L, the zincic adsorption rate of HXZ-1 reached the maximum of 29.1%; the maximum zincic adsorption rate of strain HXZ-1 run up to 18.8% after cultured 10 h in concentration of zinc ions as 500 mg/L. Strain HXZ-1 had better adsorption effect and tolerance to zinc. 

Key words: zinc-resistance, 16S rRNA, Promicromonospora, growth characteristics