生物学杂志

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低温胁迫对鱼类PI3K/AKT/GSK-3β信号通路的影响

  

  1. 1. 上海海洋大学 水产与生命学院,上海 201306;2. 上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海 201306
  • 出版日期:2016-12-18 发布日期:2016-12-18
  • 通讯作者: 陈良标, 博士,教授,从事发育、分子生物学研究,E-mail: lbchen@shou.edu.cn
  • 作者简介:王金凤 ,硕士研究生,从事分子生物学研究,E-mail:jinyu1124829@163.com
  • 基金资助:
    国家“973”重点基础研究发展计划(2010CB126304);国家基金委重点项目(31130049);上海市一流学科水产基础生物学(沪教科 2012-62)

Effect of cold stress on PI3K/AKT/GSK-3β signal pathway in fishes

  1. 1. College of Aquaculture and Life; 2. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
  • Online:2016-12-18 Published:2016-12-18

摘要: 为探讨PI3K/AKT/GSK-3β信号通路在鱼类低温胁迫中的分子机制,利用3种抗寒能力不同的鱼类:斑马鱼(Danio rerio)、罗非鱼(Oreochromis niloticus)和草鱼(Ctenopharyngodon idellus),对其进行低温胁迫(8℃),监测这3种鱼鱼鳃中PI3K/AKT/GSK-3β信号通路中蛋白表达情况。结果表明:1)在相同低温胁迫下,3种鱼运动能力为:草鱼 > 斑马鱼 > 罗非鱼;2) Western blot结果表明:在罗非鱼中,低温抑制了p-PTEN蛋白的表达;诱导了AKT、p-AKT(Ser 473)和p-GSK-3β(Ser 9)蛋白的表达。在斑马鱼和草鱼中,低温都抑制了AKT和p-AKT(Ser 473)蛋白的表达;诱导了p-GSK-3β(Ser 9)蛋白的表达。尽管低温一致性诱导p-GSK-3β(Ser 9)在3种鱼中的表达,但其呈现不同的变化倍数:罗非鱼中p-GSK-3β蛋白的上升倍数显著高于其他两种鱼。低温诱导了PI3K/AKT/GSK-3β信号通路,在不同低温耐受能力的鱼中呈现出不同的表达模式,这些物种特异性的表达模式可能会影响鱼类低温下不同的耐受能力。

关键词: 低温胁迫;鱼;AKT;GSK-3&beta, ;信号通路

Abstract: To investigate the PI3K/AKT/GSK-3β signaling pathway in fish cold regulatory mechanism, three fish species, including tilapia (Oreochromis niloticus), zebrafish (Danio rerio) and grass carp (Ctenopharyngodon-idellus), were exposed to the cold temperature (8℃). They were reared at a common temperature (28℃) but had different cold tolerance (grass carp > zebrafish > tilapia).The expression of protein in the PI3K/AKT/GSK-3β pathway were examined. Results showed that the swimming performance decreased in turn: grass carp, zebrafish and tilapia. In tilapia, the expression of p-PTEN was decreased at cold temperature, whereas the expression of AKT, p-AKT(Ser 473) and p-GSK-3β(Ser 9) was increased at 8℃. Both in zebrafish and in grass carp, the expression of AKT, p-AKT(Ser 473) was repressed at 8℃, whereas the expression of p-GSK-3β(Ser 9) was induced at 8℃. Although the expression of p-GSK-3β(Ser 9) was commonly induced at cold temperature, and the increased abundance varied in the three fish species. The induction of p-GSK-3β(Ser 9) at 8℃ in tilapia was more significantly than that in other two fishes. PI3K/AKT/GSK-3β signaling pathway is involved in cold response and whose regulation vary in the fish species, which may contribute to the various cold tolerance of fishes.

Key words: cold stress, fishes, AKT, GSK-3β, signal pathway