生物学杂志

生物学杂志

• 技术方法 • 上一篇    下一篇

月桂酸淀粉酯在\[BMIM\]BF4离子液体中的酶法合成

  

  1. 1. 江苏联发纺织股份有限公司， 海安 226600； 2. 江苏省生态染整技术重点实验室， 海安 226600； 3. 江南大学 纺织服装学院， 无锡 214122
  • 出版日期:2016-08-18 发布日期:2016-08-18
  • 通讯作者: 袁久刚，博士，副教授，主要从事纺织品生物加工技术研究，Email：jiugangyuan@163.com
  • 作者简介:袁久刚，博士，副教授，主要从事纺织品生物加工技术研究，Email：jiugangyuan@163.com
  • 基金资助:
    国家自然科学基金(31300785)；江苏省企业博士创新计划（苏人才办\[2014\]27号）;江苏省产学研（BY2014023-05）

Synthesis of laurate starch through lipase-catalyzed reaction in \[BMIM\]BF4 ionic liquid

  1.  1. Jiangsu Lianfa Textile Co., Ltd, Haian 226600; 2. Key Laboratory of Eco Dyeing and Finishing, Haian 226600; 3. School of Textiles and Clothing, Jiangnan University, Wuxi 214122, China
  • Online:2016-08-18 Published:2016-08-18

PDF (PC)

147

摘要： 为了改善淀粉的疏水性能，采用固定化脂肪酶CALB在\[BMIM\]BF4离子液体中对淀粉进行了月桂酸酯化改性。首先，对反应后得到的产物结构进行了核磁分析，结果显示月桂酸在脂肪酶的催化作用下成功对淀粉进行了改性。其次，研究了不同因素对产物取代度的影响，结果表明月桂酸淀粉酯的较优制备条件为：脂肪酶用量0.2 g、月桂酸/AGU摩尔比=3∶ 1、反应温度60℃、反应时间4 h。最后，对月桂酸接枝后淀粉的疏水性能进行了测试，结果显示淀粉的润湿接触角明显提高，而且随着取代度的增加，疏水性也越高。

关键词: 脂肪酶, 淀粉, 月桂酸, 离子液体

Abstract: In order to improve the hydrophobilicity of raw starch, lauric acid was grafted on starch through CALB lipase-catalyzed reaction in \[BMIM\]BF4 ionic liquid. Firstly, the structure of modified starch was tested with 1H NMR, it showed that the lauric acid was successfully grafted on starch. Secondly, the effect of different reaction conditions on the DS values of modified starch was studied in detail. Results showed that a better synthesis condition for laurate starch was as follows: lipase dosage 0.2 g, lauric acid/ AGU=3:1, temperature 60℃，time 4 h. Finally, the hydrophobilicity of modified starch was tested through water contact angle. Results showed that the hydrophobicity of modified starch was significantly improved and increased with DS values. 

Key words: lipase, starch, lauric acid, ionic liquid