生物学杂志 ›› 2024, Vol. 41 ›› Issue (4): 71-.doi: 10.3969/j.issn.2095-1736.2024.04.071

• 研究报告 • 上一篇    下一篇

盐胁迫对桔梗生理生化指标及总皂苷积累的影响

张美茜, 于 娟, 曹 阳, 麻剑南, 包晨丽, 王晓琴, 贾 鑫   

  1. 内蒙古医科大学 药学院, 呼和浩特 010110
  • 出版日期:2024-08-18 发布日期:2024-08-14
  • 通讯作者: 王晓琴,博士,教授,主要从事中蒙药活性成分分布规律及质量控制研究,E-mail:nywangxiaoqin@163.com;贾鑫,博士,副教授,主要从事中蒙药资源与栽培等研究工作,E-mail:jiaxin198761231@163.com;王晓琴和贾鑫为共同通信作者
  • 作者简介:张美茜,研究生,研究方向为中药资源与开发,E-mail:627664941@qq.com
  • 基金资助:
    国家自然科学基金地区科学基金项目(81960687)

Effects of salt stress on physiological and biochemical indicators and total saponin accumulation of Platycodon grandiflorus(Jacq.)A.DC.

ZHANG Meixi, YU Juan, CAO Yang, MA Jiannan, BAO Chenli, WANG Xiaoqin, JIA Xin   

  1. School of Pharmacy, Inner Mongolia Medical University, Hohhot 010110, China
  • Online:2024-08-18 Published:2024-08-14

摘要: 为探究桔梗对盐胁迫的响应,以二年生桔梗为试验材料,通过盆栽的方式,设置对照组(CK,正常浇水)、低浓度盐胁迫(S1,200 mmol/L NaCl)、中浓度盐胁迫(S2,400 mmol/L NaCl)和高浓度盐胁迫(S3,600 mmol/L NaCl)等4个处理组,测定不同浓度盐胁迫处理下桔梗幼苗的生物量、生理生化指标及总皂苷含量。结果表明:随处理时间的延长,盐胁迫会抑制桔梗的正常生长,降低其生物量,且随盐胁迫浓度的增加抑制作用越显著;S1有利于促进桔梗叶绿素含量的增加,而S2及S3均使叶绿素含量降低;叶片相对含水量随盐胁迫浓度的增加呈下降趋势;桔梗叶片及根部抗氧化酶活性均在重度盐胁迫和处理第30天时显著降低;桔梗叶片和根部脯氨酸含量、丙二醛(MDA)含量和总皂苷含量均随盐胁迫浓度及处理时间的增加而显著升高。研究可为改善盐渍土环境下桔梗的生长发育以及提高产量和品质提供理论依据,以期为桔梗在盐碱地种植提供一定参考。

关键词: 桔梗, 盐胁迫, 生物量, 生理指标, 总皂苷

Abstract: In order to study the effect of salt stress on the growth and development ofPlatycodon grandiflorus, the experiment was conducted with two-year-oldPlatycodon grandiflorusas test material, four treatment groups including control (CK, normal watering), low concentration salt stress (S1, 200 mmol/L NaCl), medium concentration salt stress (S2, 400 mmol/L NaCl) and high concentration salt stress (S3, 600 mmol/L NaCl) were set up, the biomass, physiological and biochemical indexes and total saponins content ofPlatycodon grandiflorusseedlings were determined. The results showed that salt stress inhibited the growth ofPlatycodon grandiflorusand reduced its biomass with the increase of salt stress concentration, and S1 could promote the increase of chlorophyll content ofPlatycodon grandiflorus, S2 and S3 decreased the content of chlorophyll, the relative water content of leaves decreased with the increase of salt stress concentration, the activities of antioxidant enzymes in leaves and roots ofPlatycodon grandiflorusdecreased significantly at S3 and the 30th day after treatment. The contents of proline, MDA and total saponins in leaves and roots ofPlatycodon grandiflorusincreased significantly with the increase of salt stress concentration and treatment time. This study can provide a theoretical basis for improving the growth, yield and quality ofPlatycodon grandiflorusin salt-alkali soil.

Key words: Platycodon grandiflorus, salt stress, biomass, physiological indicators, total saponin

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