生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (4): 30-.doi: 10.3969/j.issn.2095-1736.2024.04.030

• 研究报告 • 上一篇    下一篇

PPARα激动剂橙皮油素对小鼠摄食的影响

郭莉霞1,2， 殷钟意1,2， 邓　佳1,2   

  1. 1. 重庆工商大学 环境与资源学院， 重庆 400067；
    2. 天然药物研究重庆高校市级重点实验室， 重庆 400067
  • 出版日期:2024-08-18 发布日期:2024-08-14
  • 作者简介:郭莉霞，博士，教授，研究方向为天然产物活性物质药理药效研究，E-mail:80074241@qq.com
  • 基金资助:
    重庆市自然科学基金面上项目 (CSTB2022NSCQ-MSX1420)

The influence of auraptene as an agonist of PPARα on food intake in mice

GUO Lixia1,2, YIN Zhongyi1,2, DENG Jia1,2   

  1. 1. Environmental and Resources Institute, Chongqing Technology and Business University, Chongqing 400067, China;
    2. Key Laboratory of Natural Medicine Research of Chongqing Education Commission, Chongqing 400067, China
  • Online:2024-08-18 Published:2024-08-14

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摘要： 探讨橙皮油素（Auraptene，AUR）的摄入对正常小鼠摄食的影响及其作用机制。将正常小鼠分为3组，正常对照组、AUR（2 μmol/kg）组、AUR（1 μmol/kg）组，连续灌胃给予AUR 2周，检测小鼠的累积摄食，检测血清、肝脏和下丘脑中胰岛素样生长因子（IGFs）、胰岛素样生长因子结合蛋白（IGFBPs）、下丘脑中厌食因子阿片促黑色素原（POMC）、过氧化物酶体增殖物激活受体α（PPARα）、靶基因肉毒碱棕榈酰基转移酶1A（CPT1A）mRNA和蛋白水平的变化。结果发现：AUR能够浓度依赖性地促进小鼠摄食，抑制下丘脑厌食因子POMC的表达（P<0.01），对肝脏IGFBP-1的表达有显著的促进作用（P<0.01），在血清和下丘脑中IGF-1的含量显著减少（P<0.01），但并未影响肝脏IGF-1的表达（P>0.05），同时，AUR显著增加了CPT1A在肝脏中的表达（P<0.01）。综上，AUR可显著促进小鼠摄食，其机制主要为AUR激活了PPARα，促使肝脏IGFBP-1表达增加，从而结合了血清中游离的IGF-1，使进入下丘脑的IGF-1含量减少，抑制了厌食因子POMC的表达，促进了小鼠摄食。

关键词: 橙皮油素, 摄食, 胰岛素样生长因子结合蛋白, 过氧化物酶体增殖物激活受体α, 肝脏, 大脑

Abstract: The aim of this study was to investigate the influence of auraptene (AUR) on food intake in mice and its mechanism. Mice were randomly divided to three groups including control group, 2 μmol/kg AUR group and 1 μmol/kg AUR group. AUR was continuously given by oral gavage for 2 weeks to measure the cumulative food intake of the mice, the expression of insulin-like growth factors (IGFs) in serum, liver and hypothalamus, insulin-like growth factor-binding proteins (IGFBPs) in liver, proopiomelanocortin (POMC) in hypothalamus. The levels of mRNA and protein of carnitine palmitoyltransferase 1A (CPT1A) as a peroxisome proliferator-activated receptor α (PPARα) target gene were also tested. The results showed that AUR promoted food intake in mice in a concentration-dependent manner, inhibited the expression of POMC (P<0.01), significantly promoted the expression of IGFBP-1 in liver (P<0.01), and significantly reduced the content of IGF-1 in serum and hypothalamus (P<0.01), but did not effect on IGF-1 in liver (P>0.05). Meanwhile, AUR significantly increased the expression of CPT1A in liver (P<0.01). In summary, these findings suggested that AUR could significantly promote food intake in mice. The main mechanism was that AUR promoted food intake in mice because of increasing the expression of IGFBP-1 in liver by PPARα activation, which bound the free IGF-1 in serum to decrease IGF-1 in hypothalamus and inhibit the expression of POMC.

Key words: auraptene, food intake, insulin-like growth factor-binding proteins, peroxisome proliferator-activated receptor α, liver, brain

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