生物学杂志 ›› 2022, Vol. 39 ›› Issue (2): 40-.doi: 10.3969/j.issn. 2095-1736.2022.02.040

• 研究报告 • 上一篇    下一篇

塔什库尔干羊EPO基因核苷酸多态性研究

  

  1. 喀什大学 生命与地理科学学院 新疆帕米尔高原生物资源与生态重点实验室, 喀什 844000
  • 出版日期:2022-04-18 发布日期:2022-04-18
  • 作者简介:王世锋,硕士,教授,研究方向为动物高原低氧生理及适应机制研究,E-mail: 1505143342@qq.com
  • 基金资助:
    新疆高校科研计划项目(XJEDU2021SI024);新疆自然科学基金项目(2015211B002)

The study on EPO gene nucleotides polymorphism in Tashkurgan sheep

  1. Key Laboratory of Biological Resources and Ecology of Pamirs Plateau Xinjiang Uygur Autonomous Region,College of Life and Geographic Sciences, Kashi University, Kashi 844000, China
  • Online:2022-04-18 Published:2022-04-18

摘要: 为分析新疆帕米尔高原塔什库尔干羊EPO基因多态性与高原低氧适应的关系,采用PCR、直接测序法、生物信息学方法分析EPO基因部分片段(1 382 bp)分子遗传特征、SNP、单倍型组成和群体遗传特性。通过对39个塔什库尔干羊的EPO基因核苷酸序列比对分析,获得7个单倍型、5个SNP位点,其中Hap 3为优势单倍型(占64%),SNP/C1991A可能与绵羊低氧适应的分子机制有关。结果显示绵羊EPO基因非常保守,仅在非编码区和内含子区发生突变。推测EPO可能是在基因表达或表达调控水平参与低氧适应,而不是序列本身。表明EPO基因可作为绵羊的高原低氧适应性进化研究及分子辅助选育的候选基因。研究结果将为塔什库尔干羊的遗传改良提供基础数据。

关键词: 促红细胞生成素, 高原低氧, 多态性, 适应性

Abstract: To analyze the relationship between EPO gene polymorphism and altitude hypoxia adaptation in Tashkurgan sheep in Pamir Plateau of Xinjiang, molecular genetic characteristics, SNP, haplotype composition and population genetic characteristics of part of EPO gene (1 382 bp) were analyzed by PCR, direct sequencing and bioinformatics methods. Seven haplotypes and five SNP sites were obtained by analyzing EPO nucleotide sequences of 39 Tashkurgan sheep, among which Hap_3 was the dominant haplotype (64%), SNP/C1991A may be related to the molecular mechanism of hypoxia adaptation in sheep. The results showed that EPO gene was very conservative, mutations occurred only in the noncoding region and intron region. It was speculated that EPO might be involved in hypoxia adaptation at the level of gene expression or expression regulation rather than the sequence itself. The results indicated that EPO gene could be used as a candidate gene for plateau hypoxia adaptive evolution research and molecular assisted breeding of sheep. It will provide basic data for genetic improvement in Tashkurgan sheep.

Key words: erythropoietin, plateau hypoxia, polymorphism, adaptive

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