生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (4): 71-.doi: 10. 3969 / j. issn. 2095-1736. 2021. 04. 071

• 研究报告 • 上一篇    下一篇

连作方式对蕨麻根际土壤真菌群落结构的影响

  

  1. 1.青海民族大学生态环境与资源学院, 西宁810007; 2. 青海省生物技术与分析测试重点实验室-青藏高原蕨麻研究中心, 西宁810007; 3. 西宁市气象局, 西宁810016
  • 出版日期:2021-08-18 发布日期:2021-08-18
  • 作者简介:王雅琼,博士,副教授,主要研究方向为环境生物学,E-mail: wangyaqiong727@163.com
  • 基金资助:
    青海省科技计划自然科学基金项目(2019-ZJ-978Q);青海民族大学高层次人才项目(2019XJG08)

Effects offungalcommunity structureintherhizosphere of PotentillaanserinaL.undercontinuouscroppingmodes

  1. 1. College of Eco-Environment & Resources, Qinghai Minzu University, Xining 810007, China;2. Qinghai Provincial Biotechnology and Analytical Test Key Laboratory, Tibetan Plateau JuemaResearch Centre, Xining 810007, China; 3. Xining Meteorological Bureau, Xining 810016, China
  • Online:2021-08-18 Published:2021-08-18

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摘要： 根际是微生物活动最活跃且与植物生长关系最紧密的区域之一,研究根际微生物对揭示作物连作障碍等现实问题具有重要意义。采用高通量测序方法测定不同连作年限、不同生长阶段的蕨麻根际土壤真菌群落结构。结果显示:蕨麻根际真菌群落分属5个门,14个纲,31个目,42个科和64个属,主要包括子囊菌门、担子菌门、壶菌门、球囊菌门和接合菌门,其中茎点霉属、光黑壳属、Cadophora、镰孢菌属和被孢霉属的平均相对丰度大于1%。蕨麻开花期根际土壤中的真菌多样性显著低于其他生长阶段(P<0. 05) ,连作4年的土壤真菌丰富度显著高于其他连作年限(P<0. 05) 。土壤养分对真菌群落结构的影响较大,其中速效钾的影响最大(P< 0. 05) 。研究表明,蕨麻的连作和生长周期对根际真菌群落结构有显著影响,维持真菌群落的稳定可能是蕨麻优质高效生产的途径之一。

关键词: 蕨麻, 连作, 生长阶段, 根际, 真菌群落

Abstract: The rhizosphere is one of the areas with the most active microbial activities and the closest relationship with plant growth. It is important to study the effect of plants on microorganisms to reveal the obstacles of continuous cropping. The fungal communities inrhizosphere soil ofPotentillaanserinaL. under different cropping years and different growth stages were investigated using the next-gen-eration sequencing. Results showed that fungal community covered 64 genera, 42 families, 31 orders,14 classes and 5 phylum, anddominated by five known fungal phyla, namely Ascomycota, Basidiomycota, Chytridiomycota, Glomeromycota and Zygomycota. Within the five phyla, it was found thatPhoma,Preussia,Cadophora,FusariumandMortierellawith average relative abundance were more than 1%. The samples from blooming stage accounted for the lowest diversity and they were significantly different from other growth sta-ges (P< 0. 05), and the samples from continuous cropping for 4 years were significantly higher than that of other continuous croppingyears (P< 0. 05). Soil nutrients had a greater impact on the structure of the fungal community, of which available potassium had the greatest impact (P<0. 05). The continuous cropping and growth ofPotentillaanserinaL. had significant influence on the structure ofrhizosphere fungal community, and maintaining the stability of the fungal community might be one of the ways for the high quality andefficient production ofPotentillaanserinaL.

Key words: PotentillaanserinaL. , continuous cropping, growth stage, rhizosphere, fungal community

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