生物学杂志

生物学杂志 ›› 2019, Vol. 36 ›› Issue (6): 55-.doi: 10.3969/j.issn.2095-1736.2019.06.055

• 研究报告 • 上一篇    下一篇

23份豌豆材料过氧化物酶同工酶研究 

  

  1. 1. 甘肃农业大学生命科学技术学院 甘肃省干旱生境作物学重点实验室， 兰州 730070； 2. 甘肃省农业科学院粮食作物研究所， 兰州 730070， 3. 兰州现代职业学院 农林分院， 兰州 730070
  • 出版日期:2019-12-18 发布日期:2019-12-12
  • 作者简介:王翠玲，硕士，副教授，主要从事基础生物化学与植物生理学的教学与研究，E-mail: wangcl@gsau.edu.cn
  • 基金资助:

    国家食用豆产业技术体系（CARS-09）；国家自然科学基金（31460382）


Study on peroxidase isoenzymes in 23 cultivars of Pisum sativum L. 

  1. 1. College of Life Sciences and Technology, Gansu Provincial Key Laboratory of Arid Land Crop Science, Gansu Agricultural University, Lanzhou 730070; 2. Crop Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070;3. Lanzhou Modern Vocational College-Agriculture and Forestry Branch, Lanzhou 730070, China
  • Online:2019-12-18 Published:2019-12-12

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摘要：

采用非变性聚丙烯酰胺凝胶电泳技术，对23份不同地区来源的豌豆进行过氧化物酶同工酶分析。通过对各供试材料酶谱特征和相似系数的分析，探讨供试材料间的亲缘关系。结果表明：不同地区来源的豌豆材料过氧化物同工酶在酶带数、酶量、酶活性等方面存在差异，供试材料中共出现161条酶带，14种类型，2条共有表达酶带。酶带数量在品种间差异显著，其中最多者出现8条，最少5条，Rf值范围为0.13~0.83。各个豌豆材料间的遗传相似度在0.33~1.00之间，过氧化物酶同工酶酶谱聚类分析将23份豌豆材料分为5个类群，分类结果与品种的来源和地理位置有一定的相关性，其中3号与17号材料、8号与16号材料、12号与13号材料亲缘关系相互最近；6号材料与其他材料的亲缘关系较远。


关键词: 豌豆, 聚丙烯酰胺凝胶电泳, 亲缘关系, 聚类分析, 迁移率

Abstract:

Peroxidase isozymes of pea (Pisum sativum L.) from arious geographical origins were analyzed using native polyacrylamide gel electrophoresis. Based on the zymography characteristics of cultivars tested，similarity coefficient and phylogenetic relationships among pea (Pisum sativum L.) were obtained. The results showed that there were differences in band number, the enzyme content, enzyme activity of peroxidase isoenzymes from 23 pea cultivars. A total of 161 peroxidase bands and 14 types were observed and two major enzymes were presented. There were significant differences in band number among the cultivars, with the largest number being 8 and smallest 5, and Rf value ranging from 0.13 to 0.83.The genetic similarity of 23 pea cultivars was between 0.33-0.97. The analysis of zymography bands cluster showed that 23 pea cultivars could be divided into five groups. As the result, a certain correlation was observed between the classification result, the geographic location and source of the cultivars. Specifically,No.3 and No.17 were nearest to each other as the case of No.8 and No.16; No.12 and No.13, and No.6 was farthest to all the other cultivars.


Key words: Pisum sativum L., polyacrylamide gel electrophoresis, genetic relationship, cluster analysis, mobility

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