Abstract: Low-protein diets is one of the important methods to solve the shortage of feed protein resources in China’s livestock and poultry industry. This approach is a low-protein feed pattern that is based on amino acid nutrition balance theory. It adds an appropriate amount of essential amino acids to meet the balanced amino acid nutrition required during the growth process of animals without affecting their healthy growth. However, the chemical synthesis of some limiting amino acids is complex, and the production efficiency is relatively low, while the production of amino acids through biological synthesis is of higher quality and lower cost. It is the core of low-protein diet development and application to develop high-efficiency amino acid biosynthesis technology. In this article, the biosynthetic pathways of several important amino acids in the low-protein diet model were systematically reviewed. This review pointed out that the selection of host strains and metabolic engineering modification was key to high-yield strains. The advantageous strains were introduced for the biosynthesis of amino acids, providing methods and directions for high-yield strain modification. The bioproduction of limiting amino acids in low-protein diet can better promote the healthy development of China’s animal husbandry industry.

Key words: low-protein diet, limiting amino acids, bioproduction, metabolic engineering, chassis strain