Abstract: To cultivate students’ interest and develop professionals who can adapt to industry developments, building on our team’s research in transgenic zebrafish genetic engineering, this course introduced an experimental system that features visual intuition, pathological phenomena, dynamic real-time observation, and hands-on operations. The transgenic fish expressing nitroreductase (NTR) and fluorescent proteins based on the metronidazole/nitroreductase (MTZ/NTR) cell ablation system was constructed. Treatment with MTZ eliminated fluorescent-positive cells, and pathological manifestations including brain hemorrhage, thereby generating visually intuitive results. This experiment covered all core experiments in the cell genetic engineering course. Applying these research findings to course teaching enhanced the advanced and innovative nature of undergraduate courses and provide new approaches for integrated undergraduate-graduate genetic engineering education, improving the quality and efficiency of training. This addressed the demands for experimental teaching innovation and practice. This experimental reform practice empowered the construction of double first-class disciplines, and the development of national first-class undergraduate disciplines in biomedical engineering and bioengineering, thereby advancing national first-class undergraduate programs in bioengineering.

Key words: experiment teaching of cellular genetic engineering, dynamic visualization of results, zebrafish, undergraduate and graduate integration course, teaching exploration