生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (6): 25-.doi: 10.3969/j.issn.2095-1736.2021.06.025

• 研究报告 • 上一篇    下一篇

乳腺癌细胞与巨噬细胞融合对PI3K/AKT和MAPK/ERK信号通路的影响

  

  1. 北京工业大学 环境与生命学部 生命科学与化学学院，北京 100124
  • 出版日期:2021-12-18 发布日期:2021-12-15
  • 作者简介:张丽娜，博士，讲师，研究方向为肿瘤的发生转移，E-mail: lnzhang@bjut.edu.cn
  • 基金资助:
    国家自然科学基金青年科学基金项目（81702272）；北京市自然科学基金面上项目（5202001）；北京工业大学第21届星火基金项目（XH-2020-10-02）

Effects of fusion between breast cancer cells and macrophages on PI3K/AKT and MAPK/ERK signaling pathways

  1. College of Life Science and Chemistry, Faculty of Environment and Life, Beijing University of Technology,Beijing 100124, China
  • Online:2021-12-18 Published:2021-12-15

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摘要： 为揭示细胞融合与肿瘤转移之间的分子机制，在前期已经成功构建乳腺癌细胞与巨噬细胞融合模型，发现融合细胞的增殖与转移能力显著增强的基础上，重点分析乳腺癌细胞与巨噬细胞融合对肿瘤细胞增殖转移密切相关的PI3K/AKT和MAPK/ERK信号通路的影响。Western Blot实验结果表明PI3K/AKT和MAPK/ERK信号通路的关键标志蛋白p-PI3K、p-AKT和p-ERK1/2在融合细胞中的表达显著上调，经LY294002和PD98059抑制剂阻断PI3K/AKT和MAPK/ERK信号通路后，融合细胞中p-AKT和p-ERK1/2的表达明显下降，而且融合细胞的增殖、迁移和侵袭能力均受到抑制。结果提示乳腺癌细胞与巨噬细胞融合可能通过激活PI3K/AKT和MAPK/ERK信号通路促进乳腺癌增殖与转移，这也为进一步明确细胞融合在肿瘤发生转移中的作用机制提供新的思路。

关键词: 细胞融合, 乳腺癌, 巨噬细胞, PI3K/AKT, MAPK/ERK

Abstract: In order to reveal the molecular mechanisms between cell fusion and tumor metastasis, we had successfully conducted a fusion model between breast cancer cells and macrophages. Previous studies found that the fusion hybrids exhibited significantly enhancing proliferation and metastasis abilities.Therefore, we next focused on the effects of cell fusion between breast cancer cells and macrophages on PI3K/AKT and MAPK/ERK signaling pathways closely related to tumor cell proliferation and metastasis. Western Blot results showed that the expression of the key marker proteins of PI3K/AKT and MAPK/ERK signaling pathways including p-PI3K, p-AKT and p-ERK1/2 was significantly up-regulated in the fused cells. After PI3K/AKT and MAPK/ERK signaling pathways being blocked by LY294002 and PD98059 inhibitors, the expression of p-AKT and p-ERK1/2 in the fused cells was obviously reduced, and the proliferation, migration and invasion abilities of the fused cells were inhibited. These results suggested that cell fusion between breast cancer cells and macrophages might promote the proliferation and metastasis of breast cancer by activating PI3K/AKT and MAPK/ERK signaling pathways. Our data also could provide new ideas for further understanding the mechanisms of cell fusion in tumorigenesis and metastasis.

Key words: cell fusion, breast cancer, macrophages, PI3K/AKT, MAPK/ERK

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