生物学杂志

生物学杂志 ›› 2020, Vol. 37 ›› Issue (4): 36-.doi: 10.3969/j.issn.2095-1736.2020.04.036

• 研究报告 • 上一篇    下一篇

杀鱼爱德华氏菌非编码小RNA sR082的功能研究

  

  1. 1.中国科学院海洋研究所 实验海洋生物学重点实验室, 青岛 266071;2. 中国科学院大学 地球科学学院, 北京 100049
  • 出版日期:2020-08-18 发布日期:2020-08-10
  • 通讯作者: 李墨非， 副研究员，主要研究方向为鱼类免疫与病害防控， E-mail：limofei@qdio.ac.cn
  • 作者简介:周海珍， 硕士研究生，主要研究方向为鱼类病原微生物与免疫，E-mail：haizhenzz@163.com
  • 基金资助:
    国家自然科学基金（No. 31602197）；中国科学院青年创新促进会（2017249）；中国科学院海洋研究所“汇泉学者”

Functional study of a small non-coding RNA, sR082, of Edwardsiella piscicida

  1. 1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences,
    Qingdao 266071; 2. College of Earth Science, University of Chinese Academy of Sciences, Beijing 100049, China
  • Online:2020-08-18 Published:2020-08-10

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摘要： 杀鱼爱德华氏菌(Edwardsiella piscicida)是海水养殖鱼类的重要病原菌，给我国水产养殖业造成了巨大的经济损失。前期研究发现杀鱼爱德华氏菌在不同生长环境中有多种sRNA表现出差异表达，其中一个sRNA被命名为sR082。分析了sR082在酸性条件下的表达情况，比较了sR082敲除菌株（TXΔsR082）和野生株TXD1在感染宿主细胞、组织和个体能力方面的差异。结果表明，在低pH值条件下，sR082转录水平显著提高，TXΔsR082生长能力显著降低；与TXD1相比，TXΔsR082在牙鲆细胞内的复制能力、在牙鲆组织中的侵染能力以及对牙鲆的致死能力均显著下降。这些结果表明，sR082很可能在杀鱼爱德华氏菌耐受酸性环境及感染等方面发挥重要作用。

关键词: 杀鱼爱德华氏菌, 非编码小RNA, 酸性胁迫, 毒力

Abstract: Edwardsiella piscicida is a major pathogen of marine fish in aquaculture, leading to severe economic loss. The mechanisms underline E. piscicida infection is yet to be fully understood. In our previous work, we identified multiple sRNAs with differential transcription levels under various conditions, including a sRNA termed sR082. The present study analyzed the transcription levels of sR082 of E. piscicida strain TXD1 upon acid stress. Further, with a sR082 knock-out strain designated TXΔsR082, we investigated the impacts of sR082 on the infective capacities to fish cell line, tissues and individuals, respectively. The results indicated that the transcription of sRNA was significantly upregulated under acidic condition. Compared to TXD1, the mutant strain TXΔsR082 exhibited significantly lower capacities on infecting and replicating in flounder FG cells, decreased dissemination in multiple flounder tissues, and attenuated lethality to fish. These observations suggested that sR082 likely plays important roles in response to acid stress and virulence of E. piscicida.

Key words: Edwardsiella piscicida, small non-coding RNA, acid stress, virulence

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