生物学杂志

生物学杂志 ›› 2019, Vol. 36 ›› Issue (6): 51-.doi: 10.3969/j.issn.2095-1736.2019.06.051

• 研究报告 • 上一篇    下一篇

急性邻苯二甲酸二（2-乙基）己酯（DEHP）暴露对黄颡鱼（Pelteobagrus fulvidraco）幼鱼炎性反相关基因表达的影响应 

  

  1. 宁波大学 海洋学院， 宁波 315211
  • 出版日期:2019-12-18 发布日期:2019-12-12
  • 通讯作者: 黎明，博士，副教授，主要研究方向为鱼类营养免疫学，E-mail：liming1@nbu.edu.cn
  • 作者简介:张木子，博士研究生，主要研究方向为鱼类营养免疫学，E-mail：beijingzhangmuzi@163.com
  • 基金资助:

    国家自然科学基金项目（31502176；31872541）；宁波市自然科学基金项目（2016A610083）


Effect of acute di-2-ethylhexyl phthalate（DEHP）exposure on gene expression involved in inflammatory response in juvenileyellow catfish (Pelteobagrus fulvidraco) 

  1. School of Marine Sciences, Ningbo University, Ningbo 315211, China
  • Online:2019-12-18 Published:2019-12-12

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摘要：

为了研究急性邻苯二甲酸二（2-乙基）己酯（DEHP）暴露对黄颡鱼炎性反应相关基因表达的影响，挑选初始体重为（1.80 ± 0.20）g的健康黄颡鱼幼鱼270尾，开展为期96 h的急性DEHP暴露实验。结果显示，在96 h毒性胁迫过程中，中、高浓度组实验鱼肝脏中肿瘤坏死因子（TNF）和白介素8（IL 8）基因表达量呈先升高后降低趋势；然而，高浓度组实验鱼肝脏中干扰素（IFN）基因表达量则呈逐渐降低趋势；DEHP暴露达12 h时，高浓度组实验鱼肝脏中TNF基因表达量显著高于其他组，而IL 8和IFN基因表达量在中浓度组最高；DEHP暴露分别达24、48和96 h时，中浓度组实验鱼肝脏中TNF、IL 8和IFN基因表达量均显著高于高浓度组。结果表明，黄颡鱼肝脏中炎性反应相关基因的表达与DEHP之间存在明显的剂量依赖效应，低浓度的DEHP暴露能够激活炎性反应相关基因的表达，而高浓度的DEHP暴露则会抑制其表达。


关键词: 黄颡鱼, DEHP, TNF, IL 8, IFN

Abstract:

A study was carried out to test the effects of acute di-2-ethylhexyl phthalate (DEHP) exposure on gene expression involved in inflammation response of yellow catfish. Fish (1.80 ± 0.20)g was randomly allocate triplicated to 3 groups for 96 h DEHP exposure. In medium and high DEHP concentration groups, the mRNA expression of TNF and IL 8 increased significantly at 24 h, then decreased. However, the mRNA expression of IFN decreased significantly in high concentration group. Fish in high concentration DEHP group had greater TNF mRNA expression than other groups at 12 h, but the highest mRNA expression of IL 8 and IFN was found in medium concentration group. Fish in medium concentration group had higher mRNA expression of TNF, IFN and IL 8 than fish in high concentration group at 24, 48 and 96 h. The results showed the gene expression involved in inflammatory response was dosage-depended with DEHP exposure, and low DEHP exposure up-regulated the gene expression involved in inflammatory response, but high DEHP exposure inhibited the gene expression.


Key words: yellow catfish, DEHP, TNF, IL 8, IFN

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