生物学杂志

生物学杂志 ›› 2019, Vol. 36 ›› Issue (6): 47-.doi: 10.3969/j.issn.2095-1736.2019.06.047

• 研究报告 • 上一篇    下一篇

Kisspeptin和N-氨甲酰谷氨酸对GT1-7细胞GnRH分泌的影响 #br#

  

  1. 1. 北京市农林科学院 畜牧兽医研究所, 北京 100097; 2. 甘肃农业大学 动物科学技术学院,兰州 730070; 3. 中国农业大学 动物科学技术学院, 北京 100193
  • 出版日期:2019-12-18 发布日期:2019-12-12
  • 通讯作者: 刘彦，研究员，研究方向为动物繁殖学，E-mail: liuyanxms@163.com
  • 作者简介:冯涛，博士，副研究员，研究方向为动物繁殖调控，E-mail: fengtao_gs@163.com
  • 基金资助:

    国家重点研发计划课题（2017YFD0501902）；北京市农林科学院创新能力建设专项（KJCX20170202和KJCX20170706）；国家自然科学基金（31501950）


Effects of kisspeptin and N-carbamylgulamate on GnRH secretion in GT1-7 cells #br#

  1. 1. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097; 2. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070;3. College of Animal Science and Technology, China Agricultural University, Beijing 100193， China
  • Online:2019-12-18 Published:2019-12-12

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摘要：

GT1-7细胞是体外研究GnRH分泌的理想模型。为了揭示kisspeptin和N-氨甲酰谷氨酸（NCG）对GnRH分泌活性的影响，体外培养GT1-7细胞，研究kisspeptin-10和NCG单独或组合培养对细胞增殖及GnRH分泌的影响。结果表明：kisspeptin-10和NCG单独处理均可促进GT1-7细胞增殖，组合培养对细胞增殖具有协同促进作用；kisspeptin-10单独处理可促进GnRH分泌，NCG单独处理可抑制GnRH分泌，组合处理后kisspeptin-10可补偿NCG对GnRH分泌的抑制，在一定浓度kisspeptin-10存在的条件下，NCG能够呈剂量依赖性促进GnRH的分泌。揭示了kisspeptin和NCG对GnRH分泌的促进作用。


关键词: kisspeptin-10, N-氨甲酰谷氨酸, GT1-7细胞, 促性腺激素释放激素

Abstract:

GT1-7 cell line is an ideal model to research GnRH secretion in vitro. In order to reveal the effects of kisspeptin and/or N-carbamylgulamate (NCG) on GnRH secretion, GT1-7 cells were used to evaluate the effect of kisspeptin and NCG alone or co-culture on cell proliferation and GnRH secretion. The results showed that either kisspeptin-10 or NCG could improve GT1-7 cell proliferation, co-culture with kisspeptin-10 and NCG presented a synergistic effect on cell number. Kisspeptin-10 challenge could stimulate GnRH secretion, but NCG showed an opposite effect. However, co-culture with kisspeptin-10 and NCG could compensate the decrease of GnRH secretion treated with NCG, under certain concentration of kisspeptin-10, NCG showed a dose-dependent increase of GnRH secretion. This work demonstrated the effects of kisspeptin and NCG on increasing GnRH secretion.


Key words: kisspeptin-10, N-carbamylgulamate, GT1-7 cells, gonadotropin-releasing hormone

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