生物学杂志

生物学杂志

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乙醇对谷氨酸棒杆菌重组蛋白表达的影响#br#

  

  1. 1. 江南大学 粮食发酵工艺与技术国家工程实验室， 无锡 214122; 2. 江南大学 工业生物技术教育部重点实验室， 无锡 214122; 3. 江南大学 生物工程学院， 无锡 214122
  • 出版日期:2019-08-18 发布日期:2019-08-18
  • 通讯作者: 白仲虎，教授，研究方向为生物医药蛋白的高效表达及发酵过程研究，E-mail: baizhonghu@jiangnan.edu.cn
  • 作者简介:高雄，硕士研究生，研究方向为分子微生物学，E-mail: 735984473@qq.com
  • 基金资助:
    国家自然科学基金（31570034）；江苏省自然科学基金（BK20150148）

Effects of ethanol on recombinant protein expression using Corynebacterium glutamicum

  1. 1. National Engineering Laboratory of Cereal Fermentation Technology; 2. Key Laboratory of Industrial Biotechnology, Ministry of Education; 3. School of Biotechnology, Jiangnan University, Wuxi 214122, China
  • Online:2019-08-18 Published:2019-08-18

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摘要： 谷氨酸棒杆菌是一种传统的工业微生物。为研究乙醇对谷氨酸棒杆菌表达外源蛋白的影响，将组成型表达增强绿色荧光蛋白（enhanced green fluorescent protein，EGFP）的谷氨酸棒杆菌接入含有不同浓度乙醇的培养基中培养，发现低浓度（1%）乙醇在被利用的过程中可以显著提高菌体的单位荧光强度。将乙醇与常见的几种营养物质进行了对比，发现乙醇在促进菌体生长以及蛋白表达方面表现更好。此外，根据乙醇被利用时异柠檬酸裂合酶（Isocitrate lyase，ICL）被强烈诱导的特性构建了一个表达能力较强、诱导效果较好的自诱导表达载体。

关键词: 谷氨酸棒杆菌, 乙醇, 蛋白表达, 碳源

Abstract: Corynebacterium glutamicum is a traditional industrial microorganism. In order to study the influence of ethanol on the expression of exogenous protein in Corynebacterium glutamicum, the strain that can constitutively express the enhanced green fluorescent protein (EGFP) was inoculated into the culture medium containing different concentrations of ethanol, and it was found that the unit fluorescence intensity could be significantly elevated in the process of the utilization of low concentration (1%) ethanol. Here we also demonstrated that ethanol had better performances both in cell growth and protein production compared with several common nutrients. In addition, an auto-inducible expression vector with high expression ability and inducible rate was constructed according to the strong induction of the isocitrate lyase (ICL) in the presence of ethanol utilization.

Key words: Corynebacterium glutamicum, ethanol, protein expression, carbon source

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